4K Video Downloader and aTube Catcher are both advertisement free applications that can support downloading multiple videos from website browsers: Facebook, YouTube, etc. Alternative media playersĤK Video Downloader is a free download for audio and video file formats. After the ‘Start’ button has been pressed, the blinking red frame around the predetermined area indicates that the screen capture session has begun. You can also select which folder the file is saved to.Ĭustomizable options are listed above the ‘Start’, ‘Stop’, and ‘Play’ buttons: ‘Minimize on start’, ‘Record Cursor’, etc. The default output format is WMV this can be changed in the video converter after. You can choose which audio device you wish to capture sound from for the session. Within the numerical list, choose the desired frame size for the recording.Ī cropping tool will appear to let you select the portion of your PC screen that you want to record. This operation is referred to as ‘Screen capture’ in the initial pop-up and ‘Screen Record’ in the studio suite. While enabled, the screen capture function records audio and visual aspects of the session. The aTube Catcher community can perform popular processes: screen recording and video cropping. People can view the options at the bottom of the window and use them once the download is complete: ‘Delete’, ‘Open Folder’, ‘Resize’, ‘Burn in DVD’, ‘Play File’, and ‘Clear Completed’. Press the ‘Download’ button to transfer the media to the computer or laptop. The ‘Settings’ tab allows people to enable and disable commands: ‘Keep source files after conversion’, ‘Shutdown PC when done’, etc. You just simply click on ‘Change my output folder’ and decide the new place. While aTube Catcher predetermines a folder to save the media, you are able to choose a different location by clicking on the text next to ‘Save To’. The default option within the list is ‘No Conversion’. The dropdown menu next to ‘Output profile’ let you choose different formats for video conversion.Ī multitude of conversion options are available: AVI, GIF, MOV, mpg, WMV, 3G2, 3GP, etc. In the ‘Downloader’ tab of aTube Catcher, you just paste the URL into the first box. To download YouTube videos onto a PC, you'll have to navigate to a preferred video and copy the link from that page. The process is simple and easy to retrieve video content from websites: Dailymotion, Vimeo, YouTube, etc. aTube Catcher is the best option for downloading videos from YouTube because there is no risk of personal information being taken. The software is securely installed into the PC. When people download aTube Catcher, they are able to safely retrieve multimedia content from the internet. Within the remaining pop-up, you can use the operations within the tabs: ‘Downloader’, ‘Stream Catcher’, ‘Screen Recorder’, ‘Video Converter’, etc. If you prefer to only have one screen launch on your desktop when you open the player, then you can click on the box next to ‘Don’t show again’ on the window with colored options. The functions are duplicated in the larger window. A bright and intuitive user interface will appear with options: ‘Download Videos’, ‘Video Converter’, ‘Screen Capture’, ‘Convert Videos to MP3’, ‘Find Videos’, DVD/Blu-Ray/VCD Creator’, ‘Resize Video’, ‘Audio Record’, and ‘Music’. Once the default settings have been saved, two windows appear. Upon completing the installation process, users are able to choose their preferred language: English, Spanish, etc. While the app is only available on Windows PC computers and laptops, the content that is captured on the devices can be exported to Android, GPS, iPad, iPhone, iPod, MP4, and PSP devices – along with other versions of computers and handheld systems. Is aTube Catcher free?ĭeveloped and released by DsNET, aTube Catcher is a free application for the community to use. The location that the media uploads to can be determined directly on the ‘Download Videos’ screen. aTube Catcher pulls data from the provided link to transfer that directly to a specified folder on the PC. Ensure that the files are certified safe before uploading them. While the additional apps may not be malicious, you should skip them to be safe.Īudio and video content from the internet can be risky to download. Once the commands are unticked, you can click ‘Next’ and navigate to ‘Skip this and all remaining offers’ to avoid the other foreign programs. If the aTube Catcher community does not want to have external software on their PC devices, then you can uncheck the boxes that install the third-party software. You'll want to make sure to be careful while downloading aTube Catcher because if you click through the pages too quickly, then you may accidentally install other apps during the process. After accepting the license agreement within the installation process, a window prompting you to download third-party freeware will arise. ATube Catcher is safe to install on PC computers and laptops.
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The confirmatory HIV test employs a western blot to detect anti-HIV antibody in a human serum sample. It is also used in medical diagnostics, e.g., in the HIV test or BSE-Test. The western blot is routinely used for verification of protein production after cloning. In addition, applying a dilution series of a purified protein of known concentrations can be used to allow a more precise estimate of protein concentration. A semi-quantitative estimation of a protein can be derived from the size and color intensity of a protein band on the blot membrane. It is used as a general method to identify the presence of a specific single protein within a complex mixture of proteins. At least 8–9% of all protein-related publications are estimated to apply western blots. The western blot is extensively used in biochemistry for the qualitative detection of single proteins and protein-modifications (such as post-translational modifications). Western blot HIV test where first two strips are negative and positive controls followed by actual tests Between 19 "it has been mentioned in the titles, abstracts, and keywords of more than 400,000 PubMed-listed publications" and may still be the most used protein-analytical technique. Jaime Renart, Jakob Reiser, and George Stark in Stanford, and by Harry Towbin, Theophil Staehelin, and Julian Gordon at the Friedrich Miescher Institute in Basel, Switzerland. Neal Burnette in 1981, although the method itself was independently invented in 1979 by Similarly, detection of RNA is termed as northern blot. The name western blot is a play on the Southern blot, a technique for DNA detection named after its inventor, English biologist Edwin Southern. Other related techniques include dot blot analysis, quantitative dot blot, immunohistochemistry and immunocytochemistry, where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA). The secondary antibody is visualized through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein. A secondary antibody is added which recognizes and binds to the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A synthetic or animal-derived antibody (known as the primary antibody) is created that recognizes and binds to a specific target protein. Western blot technique uses three elements to achieve its task of separating a specific protein from a complex: separation by size, transfer of protein to a solid support, and marking target protein using a primary and secondary antibody to visualize. Besides detecting the proteins, this technique is also utilized to visualize, distinguish, and quantify the different proteins in a complicated protein combination. The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. This trial is absolutely free and there are no strings attached.ġ Rev. You'll get a no-obligation 7-day FREE trial during which you can read all of our helpful tax saving tips from the last two months. If you are not yet a subscriber, CLICK HERE. If you can find $10,000 in new deductions, you pocket $2,400. That puts the two of you in the 24 percent federal income tax bracket. You and your spouse have taxable income of $210,000. Why? That’s where you start to pocket cash when you find a new or additional tax deduction.Įxample: You are married. When looking at your federal income tax bracket, pay attention first to your last bracket. Married Individuals Filing Separate Returns Unmarried Individuals (other than surviving spouses and heads of households) $154,793.50 plus 37% of the excess over $518,400 The top marginal income tax rate of 37 percent will hit taxpayers with taxable income of 523,600 and higher for single filers and 628,300 and higher for married couples filing jointly. Married Individuals Filing Joint Returns, & Surviving Spouses Find out your 2020 federal income tax bracket with user friendly IRS tax tables for married individuals filing joint returns, heads of households, unmarried individuals, married individuals filing separate returns, and estates and trusts. Key started the song with "I'm scared of getting older" - same Key, same. Just as the title suggests, this dreamy and beautiful song reminds us of the times when we were kids, innocent and idolizing invincible superheroes. This song, in particular, has lyrics written like how a child would write and express themselves so straightforward and simple. No one's quite able to express this longing for childhood as well as LUCY does. Their songs are always so emotion-filled and written with such heartfelt love and passion. Of course, The Rose won't reuse the cliche "live like there's no tomorrow," but instead sings, "don't live your life like you have a thousand years." Again, reminding us to live our best life to the fullest. If there was one thing I could tell my younger self, it was to enjoy the moment and not dread wanting to grow up so fast because, as ONEWE sings, "we will miss the present when we grow up." We will grow up eventually, but we can never relive our youth, so live your present to the fullest! The song is about a relationship you had when you were younger and immature and the regrets and realization you had about it as you grew up. Their beautiful and emotional voice just touches your heart more than ever. Day6 reminded me of those times, and I really do wanna go back. No one warned me that adulting would be this hard, and it's only become more difficult after I turned 25 these songs now have a much deeper meaning for me.ĭon't we all just wish we could go back to the times when everything was simpler? When our only concern was to choose whether to hang out with friends or to go home after school. As we just stepped into the new year, I'm starting to be reminded that I'll be a year older in a few months, and I can't help but feel a bit nostalgic for my younger days. The trainer also prompted her to touch her nose, bow thank you and do a simple dance. Though such a sight would be cringe-worthy in many other locales, it seemed to delight visitors who roared with laughter on Wednesday as the chimpanzee, one of two at the zoo, sat puffing away as her trainer egged her on. If a lighter isn’t available, she can light up from lit cigarette if one is tossed her way. Thrown a lighter by a zoo trainer, the chimpanzee lights her own cigarettes. They insist, however, she doesn’t inhale. The zoo actually dates back to 1959, when Kim Il Sung, the nation's first leader and the grandfather of Kim Jong Un, ordered it built on the outskirts of the city.Īccording to its official history, the zoo started off with only 50 badgers.PYONGYANG, North Korea > Pyongyang’s newly opened zoo has a new star: Azalea, the smoking chimpanzee.Īccording to officials at the newly renovated zoo, which has become a favorite leisure spot in the North Korean capital since it re-opened in July, the 19-year-old female chimpanzee, whose name in Korean is “Dallae,” smokes about a pack a day. Renovations for the new zoo began in 2014, as part of North Korean leader Kim Jong Un's efforts to create more modern and impressive structures and leisure centers around the capital. The zoo also has performances featuring other animals trained to do tricks, including a monkey that slam dunks basketballs, dogs trained to appear as though they can do addition on subtraction on an abacus and doves that fly around and land on a woman skating on an indoor stage. The zoo is pulling in thousands of visitors a day with a slew of attractions ranging from such typical fare as elephants, giraffes, penguins and monkeys to a high-tech natural history museum with displays showing the origins of the solar system and the evolution of life on Earth.Īnother of the most popular attractions that might come as a surprise to foreign visitors is the dog pavilion, which has everything from German shepherds to Shih Tzus. Though such a sight would draw outrage in many other locales, it seemed to delight visitors who roared with laughter on Wednesday as the chimpanzee, one of two at the zoo, sat puffing away as her trainer egged her on. If a lighter isn't available, she can light up from lit cigarette if one is tossed her way. They insist, however, she doesn't inhale. PYONGYANG, North Korea (AP) - Pyongyang's newly opened zoo has a new star: Azalea, the smoking chimpanzee.Īccording to officials at the newly renovated zoo, which has become a favorite leisure spot in the North Korean capital since it re-opened in July, the 19-year-old female chimpanzee, whose name in Korean is "Dallae," smokes about a pack a day. Samsung Data Recovery is a professional data recovery app which allows you to recover deleted photos from SD card on Android phone without backup. How to Recover Deleted Photos from SD Card on Android Phone without Backup (Recommended) SHAREit Review - Everything to Know: Price, Features Pros, Cons, Interfaceįast Recover Deleted TikTok Videos from Android/iOS Smartphones and Computers Part 2. After that, you can use the professional photo recovery app below to recover deleted photos from SD card on Android. That is to say, in order to successfully recover deleted photos from SD card on Android, you'd better switch off the Wi-Fi/cellular data on Android and power your mobile off. That is because the deleted photos on the Android SD card are just marked as replaceable or invisible in system, and once new data generates, it will erase the lost photos permanently. If you have lost photos from Android SD card, don't panic! The lost photos are possible to recover with the help of a professional photo recovery app before new data generates on your Android SD card. Abrupt removal of SD card from the computer when the transfer process is not complete.SD card corrupted or became inaccessible without any reason.Unintentional SD card formatting without photo backup beforehand.Accidental deletion of some important photos while deleting some unwanted photos.There are many reasons why you lose photos from the SD card. Something You Need to Know before You Recover Deleted Photos from SD Card on Android Phone Useful Tips to Prevent Android Photos from Losing on SD Card Again How to Restore Deleted Photos from SD Card on Android Phone with Backup ‘He violated my body and pierced my soul with a dagger. ‘I will never forget that first time, that moment,’ she says. She was raped repeatedly during the seven years she was held captive, eventually escaping when a door was accidentally left unlocked.įuelled by cocaine and alcohol – and often Viagra – Gaddafi abused her horribly. When she tried to run out, the women soldiers grabbed her and flung her back on the bed. When she was shoved into Gaddafi’s room, to her horror he was lying naked on the bed. She was dressed in a G-string, forced into a low-cut gown and had thick make-up plastered on her face. The girl was stripped, given a blood test and shaved of all but her pubic hair. Once there, he barked at his women soldiers: ‘Get her ready.’ Instead, she was driven at high speed to Gaddafi’s lair. The next day three woman dressed in military uniform arrived telling her parents she was needed to present more flowers. When he patted her head afterwards, in an apparently paternal gesture, she thought she had pleased the man she and her fellow Libyans were forced to call ‘the Guide’. She had been chosen to present the colonel with a bouquet when he toured her school in his home town of Sirte on the Mediterranean coast, 350 miles east of Tripoli. She had been left for dead.’Įven today, the Libyan people are afraid to speak openly about Gaddafi’s depravity, fearing reprisals from his former henchmen.īut one woman – who was repeatedly raped by the despot over seven years from the age of 15 – has anonymously spoken of how he terrorised and abused her. Another was found three months later, cut, raped and lying in the middle of a park. ‘One just disappeared and they never found her again, despite her father and brothers searching for her. ‘The girls he wanted would be rounded up and sent to him,’ she said. She told her mother how the community around the university lived in fear when a visit from the colonel was announced. The teacher also feared for her own daughter who was attending Tripoli University. They had no conscience, no morals, not an iota of mercy even though she was a mere child.’ ‘They would simply take the girl they wanted. One teacher at a Tripoli school recalled how the girls were all very young. If their families tried to keep them from Gaddafi’s clutches, they were gunned down. Within hours his private bodyguards would round up those chosen and kidnap them. Before leaving he would pat those he had ‘selected’ on the head. Gaddafi’s modus operandi was to tour schools and universities where female students were invited to his lectures.Īs he spoke before his hushed audience, he would silently scan the room seeking out attractive girls. Other young victims were so badly abused that they were dumped in car parks and on waste ground, and left to die. In pursuit of an evil wizard, Kai is left with little choice but to assist her throughout the labyrinthine underground dungeons that lie beneath the tavern.'Sexual deviant': Colonel Gaddafi kept hundreds of girls as sex slaves during his years at the helm of Libya, but also kept a 'harem' of young boys One fateful night though, a tipsy princess stumbles through the door an encounter that would change his life. However, he has yet to make any progress in the mysterious, timeless tavern he serves at. This hot mess just won't stop.! Play responsibly.īarkeep "Kai" has but one goal to become a legendary 'Master Bartender'. Can you stomach to go spelunking in the cursed booze-dungeon of Yashumugi? This game presents a whole new harem of charming maidens and new, exotic dungeons to explore. Oh snap! Here comes the second installment of the lewd-but-cute dungeon-crawler "Girls and Dungeons"!įeaturing all new JRPG shenanigans and naughty features for newcomers and connoisseurs alike. In the build-up to some holidays, stores receive festive makeovers. Shelf stockers and warehouse attendants work during the night to ensure the store floor is fully stocked and clean before opening. RetailĮven when the stores are closed, people work behind the scenes preparing for the next trading day. The roads are often quieter for truck drivers at night, allowing them to reach their destination quicker.įlights depart throughout the day, so flight crew and airport staff are needed to check passengers in and get them to their destination safely. Transportationīus and taxi drivers, flight attendants, airport staff and truck drivers all work swing shift hours. Police, firefighters and correctional workers are needed 24/7 to help maintain order and offer help and support where needed. Similar to healthcare, incidents can happen at any time. Therefore, paramedics, doctors, and nurses are needed to work both day and night shifts.Īdministrative and cleaning staff may also be needed outside of typical business hours to help keep the medical facilities running efficiently. Medical emergencies can occur at any time. The industries where swing shift hours are common are: Healthcare What Industries Commonly Have Swing Shift Hours? This article will discuss the industries with swing shift hours to help you understand your role, the advantages and disadvantages of working on a swing shift schedule, and how to excel in these positions. Your employer could implement swing shift hours to increase productivity or meet their customers' needs, depending on the industry you work in. These shifts are designed to streamline the operations of an organization so the work can continue 24/7. Swing shift hours are common in organizations that need employees to work throughout the day and night.Īn employer could offer you a choice of the different shifts however, your schedule is dictated by the organization's operational needs. shift one day and something different the next. to 5:00 p.m., five days a week, you could be working on a 4:00 p.m. If you apply for a job that mentions swing shift hours, you will almost certainly be working outside of regular business hours. If you are NOT a CAL FIRE employee, or if you are an employee who wants to help support the causes, check out the Fundraising Tab and support various Firefighter Organizations. The new CAL FIRE Travel Mug is available for purchase now. We appreciate your patience and understanding. As soon as inventory is replenished we will ship any backordered items. But sometimes we are just out of inventory. We are doing our best to provide you with the highest level of service and prompt order turn around. As such, many items are presently not in the supply chain. Production has been limited and in some cases ceased due to governmental restrictions on operations as well as social distancing. Developed using recommendations from the Department of Homeland Security Wildland Firefighter PPE clothing system program, this line was meticulously purpose. Please keep in mind that the COVID-19 situation is affecting our suppliers as well. We look forward to serving you.ĭUE TO THE ONGOING LABOR SHORTAGE ISSUES AND PRIOR STAFF COMMITTMENTS, WE WILL BE CLOSING FRIDAYS AT 1:00 PM UNTIL FURTHER NOTICE. COVID-19 UPDATE - The office will be open for Will Call orders from Monday thru Thursday 8:30am to 4:00pm. NEW HIRES - may provide their letter of authorization issued by the department. : Cal Fire Tshirt 97-144 of 197 results for 'cal fire tshirt' RESULTS Price and other details may vary based on product size and color. CA Penal Code Section 538ee provides that any person purchasing a fire uniform must provide evidence of employment. CAL FIRE Duty Apparel is available to Employees of CAL FIRE only. Pacific Gateway Hotel at Vancouver Airport is one of the most popular hotels in Richmond. In Richmond, chain hotels such as Ramada are one of the top choices among travelers. Travelers who favor this brand can choose to stay there to enjoy the luxury services provided by Ramada. In Richmond, there are hotels under the Ramada brand. There are many well-known chain hotels in Richmond. There are 5 two-star hotels in Richmond at an average price of 124 USD per night. There are 10 three-star hotels in Richmond at an average price of 135 USD per night. There are 9 four-star hotels in Richmond at an average price of 183 USD per night. There are 1 five-star hotels in Richmond at an average price of 247 USD per night. There are a variety of hotels to meet the needs of different types of travelers. The local average price is 153 USD per night. On, there are 78 recommended hotels in Richmond. If you travel to Canada, visiting Richmond is a good choice.įor those traveling for business and tourism, Vancouver International Airport is the preferred choice when visiting Richmond. If you want to stay at the best hotel in Richmond, is here to help! You can plan a one or two day trip to Richmond. New and popular hotels in Richmond recommended by. She had denied ever calling my room to confir Upon speaking in person with Nini, my wife recognized her voice on the phone. It was only two girls working, Nini and Monica at front desk. Nini did not provide any help or resolution, she had dismissed my wife’s needs as a guest and failed to provide her with adequate information for her confirmed bookings at Versante Spa. Nini then told my wife there wasn’t a shuttle coming and pointed at the door and told her to walk. When my wife told her we had received a phone call from her (at 315PM) to confirm our shuttle, Nini had denied making any calls to our room. Ultimately, Nini said my wife wasn’t booked for a shuttle. Nini then told her there was no shuttle that goes to the spa and it only goes to YVR. My wife informed her about her appointment at the spa and shuttle booking, which should have made it obvious to Nini that the booking was for the Versante Spa further away. He then took my wife to the receptionist who was working at the front, named Nini, and asked about the shuttle booking. My wife asked the Valet who was there and he asked her for her name and said it was not ticketed. At 345PM my wife went downstairs to meet the shuttle that we had booked the day before for my wife’s spa appointment. We then told her it was confirmed for 345PM. Nini asked to confirm if we had booked a shuttle for 330PM. On Wednesday, August 31st at 315PM we received a call from our hotel room (1404) from Nini, the front desk receptionist. Manager Yasin was the one who had confirmed our shuttle booking for the spa the next day at 3:45PM. On Tuesday, August 30th we booked for a hot stone massage at Spa Versante and confirmed our booking for a shuttle the next day at 3:45PM. What had occurred in your hotel with the staff members that you hired to take care of your guests was unbelievably heartbreaking and I do not wish for anyone to go through what my wife and I had to witness. I would like to mention there were a few notable staff members that were exceptional and compassionate, and it’s a shame that this experience would reflect poorly on the hard working individuals who really tried to make our stay memorable. It was a betrayal of trust from the Versante hotel staff and what Versante portrayed their company’s values to be. What we thought was going to be one of the most relaxing stays through our hotel experience quickly turned into a nightmare at the Versante hotel. We booked the penthouse suite on the 14th floor and loved it so much that we cancelled our reservations at the Fairmont hotel, just so we can extend our stay before we go to the airport for our family trip on the 6th. My wife and I booked our stay at the Versante hotel, originally from Monday, August 29th until Saturday, August 3rd. (My name is anonymized to protect my identity for this review) This review was delayed due to an ongoing police investigation about our stay here in the Versante Hotel. |
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